Diluting of the cell sample is used to get a cell concentration which lies within the counting range1 of the cell counter.
In case you are using a diluted sample, we offer the option to fill this in before starting your cell count. To calculate your dilution factor, click Yes next to the sample dilution option. Fill in the amount of dilution used in the form of (sample):(dilution). In the following image a dilution of 1 part sample and 10 parts dilution is used and will result in a dilution factor of (1+10)=11.
The calculation of the dilution factor is (sample + dilutant)
In case of using a viablity dye the sample is diluted by a factor 2 or (1:1) and the final dilution factor is multiplied by 2.
The following image contains a calculation example of the dilution factor. In the left (1:4) dilution is shown and in the right (1:1) trypan blue. The final dilution factor is (1+4)*2=10
1 The range limit of the Corning Cell Counter is: 5 × 104 to 1 × 107 cells/mL