When a problem with the CytoSMART® Exact FL occurs, an orange notification will appear with a message. One or multiple of the following messages can appear:
This message appears when there is no connection to the device. Check if the USB cable is properly connected to the device and the laptop. If the warning still appears after checking, please try a different USB 3.0-port of the laptop.
This message appears when the laptop is not charging. Please connect the laptop to the power supply.
This message appears when the software is started but the laptop has no internet connection. Please make sure to connect the laptop to internet via WiFi or cable.
When you are trying to access the channel settings panel while the device is sleeping this message appears. Please tap or click on the screen to wake up the device. After the device has woken up, you can make adjustments to the channel settings.
This message appears in case you try to access the channel settings panel while the device is not connected. Please make sure the USB cable is properly connected to the device and laptop.
This message appears when the exposure time of the fluorescent channel you have chosen is longer than 1.1 seconds. Any changes that you apply to the channel settings will take the duration of the chosen exposure time before they can be observed in the live view.
When the live view of one of the fluorescent channels is turned on for a long time the LED overheats and shuts down to prevent the LED from burning out. Please wait a minute for the LED to cool down to a safe temperature before turning it on. You can still adjust the settings of the other fluorescent channel or the brightfield channel in the meantime, but you need to turn on the LED of the overheated channel manually.
This message appears in the results page when the cell concentration of the sample is below 5.0*104 cells/mL. This is below the operating range of the CytoSMART® Exact FL. To resolve this, pellet the solution by centrifugation and resuspend the cells in a smaller volume.
Another cause might be that the bulk sample solution has been left on the bench for some time. Cells have then gravitated towards the bottom of the vial. By pipetting the suspension up and down before loading the hemocytometer, you should obtain a homogenous cell suspension.
This message appears in the results page when the cell concentration of the sample is above 1*107 cells/mL. Dilute you cell suspension to obtain a lower concentration and count again.
You might observe one of the following problems in your images. For accurate results, exclude those images from the analysis or redo the counting.
Cells can aggregate into clumps, leading to false results when analyzed. The algorithm is trained to recognize individual cells within cell clumps properly, but it is important to ensure correct focus.
Bubble can be recognized as big black circles. Bubbles can lead to false results, as they influence the distribution of cells and the volume inside the counting chamber. The cause for bubbles is often due to incorrect pipetting. Therefore, try to prevent having air bubbles in your sample and prevent including air during loading the sample into the chamber.
This may be caused by not having properly cleaned the hemocytometer. To resolve this, properly clean the hemocytometer before loading a new sample.